Ecotoxicology
& Risk Assessment
MARINNOVA offers an extensive choice of tools to support regional, national, and private companies obligations such as the Water Framework Directive (WFD), the European Marine Strategy Framework Directive, OSPAR, REACH, etc.
Toxicity tests
- Code
- Analysis
- Method
- Price per
assay (EURO) - Sample volume/mass
- Embryo toxicity tests
- CS1
- Embryo toxicity test with freshwater zebrafish (Danio rerio)
- Full embryo development assay according to OECD test n. 212
- On request
- Variable. Single compounds, field samples, effluents
- CS2
- Embryo toxicity test with sea urchin (paracentrotus lividus)
- Embryo toxicity test according to EPA standard test in US and Canada
- On request
- Variable. Single compounds, field samples, effluents
- CS3
- Embryo toxicity test with marine fish Scophthalmus rhombus
- Embryo toxicity test
- On request
- Variable. Single compounds, field samples, effluents
- Toxicity tests with aquatic invertebrate, microalgae, in vitro cultures
- CS4
- Daphnia sp. Acute Immobilisation Test
- OECD test n. 202
- On request
- Variable. Single compounds, mixtures, field samples, wastewaters, effluents
- CS5
- Daphnia magna Reproduction Test
- OECD test n. 211
- On request
- Variable. Single compounds, mixtures, field samples, wastewaters, effluents
- CS6
- Freshwater Alga and Cyanobacteria, Growth Inhibition Test
- OECD test n. 201
- On request
- Single/mixture compounds
- CS7
- In vitro tests with cell cultures
- Hepatocytes primary cultures
- On request
- Variable. Single compounds, mixtures, field samples, wastewaters, effluents
Biotoxins assays
- Code
- Analysis
- Method
- Price per
sample (EURO) - Sample volume/mass
- BIOT1
- Quantification of hepatotoxins (microcystins) in water samples or organisms *
- ELISA with policlonal antibodies
- On request
- 100 ml
- BIOT2
- Quantification and identification of microcystins and cylindrospermopsin *
- HPLC with Diode Array detector (allows to discriminate the different toxins)
- On request
- 1000 ml
- BIOT3
- Detection and quantification of neurotoxins (anatoxin-a) *
- HPLC with fluorescence detector
- On request
- 1000 ml
Screening assays
- Code
- Analysis
- Method
- Price per
sample (EURO) - Sample volume/mass
- CS8
- Biomarker of genotoxicity (multiple fish species)
- ENA (erythrocyte nuclear abnormality assay), Comet assay
- On request
- 5 ul of blood. Adequate for testing field, effluent samples and single/mixture compounds
- CS9
- Biomarker of exposure to aryl hydrocarbon receptor (AhR) agonists (PAHs, PCBs, ect.)
- EROD activity
- On request
- 50 mg liver. Adequate for testing field, effluent samples and single/mixture compounds
- CS10
- Biomarker of estrogenic chemical exposure (fish)
- Vitellogenin gene expression (real time PCR)
- On request
- 20 mg liver. Adequate for testing field, effluent samples and single/mixture compounds
- CS11
- Biomarker of organotin exposure (snails)
- Imposex in marine gastropods
- On request
- 30 females. Adequate for testing field, effluent samples and single/mixture compounds
- CS12
- Citotoxicity and cell viability assays
- Neutral red retention, Trypan blue exclusion assay
- On request
- Variable. Fish hepatocytes, invertebrates digestive gland or haemolymph
- CS13
- Biomarker of PAHs exposure (fish)
- Fixed Wavelength Fluorescence (FF)
- On request
- 5 ul of bile. Adequate for testing field, effluent samples and single/mixture compounds
- CS14
- Biomarkers of exposure to oxidatants (metals, PAHs, PCBs, etc.)
- Antioxidant enzymes and oxidative damage
- Variable
- 50 mg liver
- CS15
- General biomarkers of exposure
- Enzyme activities (EROD, GST, antioxidant enzymes, etc.)
- Variable
- 50-100 mg liver
- CS16
- Biomarkers of exposure to neurotoxic compounds
- Enzyme activities (ChE enzymes)
- Variable
- 50 mg brain or muscle tissue. Adequate for testing field, effluent samples and single/mixture compounds
- CS16
- Biomarkers of exposure to neurotoxic compounds
- Enzyme activities (ChE enzymes)
- Variable
- 50 mg brain or muscle tissue. Adequate for testing field, effluent samples and single/mixture compounds
Consultancy
- Consultancy in the field of ecotoxicology, environmental monitoring and risk assessemt, focusing on current EU regulation such as the Water Framework Directive (WFD), the European Marine Strategy Framework Directive, OSPAR, REACH, etc.