Your sea of innovation

Ecotoxicology
& Risk Assessment

MARINNOVA offers an extensive choice of tools to support regional, national, and private companies obligations such as the Water Framework Directive (WFD), the European Marine Strategy Framework Directive, OSPAR, REACH, etc.

Toxicity tests

Code
Analysis
Method
Price per
assay (EURO)
Sample volume/mass
Embryo toxicity tests
CS1
Embryo toxicity test with freshwater zebrafish (Danio rerio)
Full embryo development assay according to OECD test n. 212
200
Variable. Single compounds, field samples, effluents
CS2
Embryo toxicity test with sea urchin (paracentrotus lividus)
Embryo toxicity test according to EPA standard test in US and Canada
200
Variable. Single compounds, field samples, effluents
CS3
Embryo toxicity test with marine fish Scophthalmus rhombus
Embryo toxicity test
300
Variable. Single compounds, field samples, effluents
Toxicity tests with aquatic invertebrate, microalgae, in vitro cultures
CS4
Daphnia sp. Acute Immobilisation Test
OECD test n. 202
~300
Variable. Single compounds, mixtures, field samples, wastewaters, effluents
CS5
Daphnia magna Reproduction Test
OECD test n. 211
~500
Variable. Single compounds, mixtures, field samples, wastewaters, effluents
CS6
Freshwater Alga and Cyanobacteria, Growth Inhibition Test
OECD test n. 201
~400
Single/mixture compounds
CS7
In vitro tests with cell cultures
Hepatocytes primary cultures
~400
Variable. Single compounds, mixtures, field samples, wastewaters, effluents

Biotoxins assays

Code
Analysis
Method
Price per
sample (EURO)
Sample volume/mass
BIOT1
Quantification of hepatotoxins (microcystins) in water samples or organisms *
ELISA with policlonal antibodies
150
100 ml
BIOT2
Quantification and identification of microcystins and cylindrospermopsin *
HPLC with Diode Array detector (allows to discriminate the different toxins)
100
1000 ml
BIOT3
Detection and quantification of neurotoxins (anatoxin-a) *
HPLC with fluorescence detector
100
1000 ml

Screening assays

Code
Analysis
Method
Price per
sample (EURO)
Sample volume/mass
CS8
Biomarker of genotoxicity (multiple fish species)
ENA (erythrocyte nuclear abnormality assay), Comet assay
40
5 ul of blood. Adequate for testing field, effluent samples and single/mixture compounds
CS9
Biomarker of exposure to aryl hydrocarbon receptor (AhR) agonists (PAHs, PCBs, ect.)
EROD activity
30
50 mg liver. Adequate for testing field, effluent samples and single/mixture compounds
CS10
Biomarker of estrogenic chemical exposure (fish)
Vitellogenin gene expression (real time PCR)
50
20 mg liver. Adequate for testing field, effluent samples and single/mixture compounds
CS11
Biomarker of organotin exposure (snails)
Imposex in marine gastropods
100
30 females. Adequate for testing field, effluent samples and single/mixture compounds
CS12
Citotoxicity and cell viability assays
Neutral red retention, Trypan blue exclusion assay
30
Variable. Fish hepatocytes, invertebrates digestive gland or haemolymph
CS13
Biomarker of PAHs exposure (fish)
Fixed Wavelength Fluorescence (FF)
30
5 ul of bile. Adequate for testing field, effluent samples and single/mixture compounds
CS14
Biomarkers of exposure to oxidatants (metals, PAHs, PCBs, etc.)
Antioxidant enzymes and oxidative damage
Variable
50 mg liver
CS15
General biomarkers of exposure
Enzyme activities (EROD, GST, antioxidant enzymes, etc.)
Variable
50-100 mg liver
CS16
Biomarkers of exposure to neurotoxic compounds
Enzyme activities (ChE enzymes)
Variable
50 mg brain or muscle tissue. Adequate for testing field, effluent samples and single/mixture compounds
CS16
Biomarkers of exposure to neurotoxic compounds
Enzyme activities (ChE enzymes)
Variable
50 mg brain or muscle tissue. Adequate for testing field, effluent samples and single/mixture compounds

Consultancy

Consultancy in the field of ecotoxicology, environmental monitoring and risk assessemt, focusing on current EU regulation such as the Water Framework Directive (WFD), the European Marine Strategy Framework Directive, OSPAR, REACH, etc.